What is the principle of biuret method?
Biuret protein assay is based on the principle that copper ions (Cu2+) react with peptide bonds in proteins, forming a complex that absorbs light at a specific wavelength. The biuret reagent used in the assay contains copper ions that react with the peptide bonds in the protein to form a purple-colored complex.
Biuret protein assay is based on the principle that copper ions (Cu2+) react with peptide bonds in proteins, forming a complex that absorbs light at a specific wavelength. The biuret reagent used in the assay contains copper ions that react with the peptide bonds in the protein to form a purple-colored complex.
