What is the main problem with the acridine orange stain?
Furthermore, acridine orange staining is particularly sensitive to pH levels, and, because of this, some difficulty may be experienced in obtaining the correct colours. In addition, the procedure does not differentiate between single-stranded DNA and RNA, using the stain alone.
Furthermore, acridine orange staining is particularly sensitive to pH levels, and, because of this, some difficulty may be experienced in obtaining the correct colours. In addition, the procedure does not differentiate between single-stranded DNA and RNA, using the stain alone.