Is adenylation reversible?
As the adenylation reaction is reversible, an excess of radio-labeled [32P]PPi is added to drive the backward-reaction, generating radio-labeled [32P]ATP. This radioactive molecule can then be adsorbed onto active charcoal and quantified by scintillation counting.Jan 26, 2023
As the adenylation reaction is reversible, an excess of radio-labeled [32P]PPi is added to drive the backward-reaction, generating radio-labeled [32P]ATP. This radioactive molecule can then be adsorbed onto active charcoal and quantified by scintillation counting.Jan 26, 2023
